国家神经系统疾病临床医学研究中心数据管理与共享系统
Data Management And Sharing System of China National Clinical Research Center for Neurological Diseases
Extracellular ASC exacerbated the recurrent ischemic stroke in an NLRP3-dependent manner
第一作者：He Xiao-Fei
通讯作者：Pei Zhong
基本信息
- 负责人：He Xiao-Fei
- 数据来源： OTHERS
- 论文编号：OTHERS20190781
- 发表时间：2019-06-19
2019-06-19
已发表文章
发表杂志：Journal of cerebral blood flow and metabolism
发表类型: Original Article
影响因子(IF): 6.2
PMID: 31216943 Pubmed原文链接 全文下载
主要研究背景及目的
方法
Next, using a photothrombotic mouse model of recurrent stroke, we found that recurrent strokes increased the activation of NLRP3, exacerbated the brain damage and the pro-inflammatory response in wild type (WT) mice, but not in NLRP3 knockout (NLRP3 KO) mice.
结论
We therefore conclude that the NLRP3 inflammasome is activated by two attacks of stroke, which act together with ASC to exacerbate recurrent strokes.
主要结果
Additionally, we found that apoptosis-associated speck-like protein containing a CARD (ASC) protein level surrounding the infarct area was comparatively increased, but that ASC specks outside of microglia in both the ipsilateral and contralateral of stroke site were decreased in NLRP3 KO mice relative to wild-type (WT) controls, and the number of ASC specks surrounding the second infarct area was positively correlated to the damage scores. Mechanistically, we found that recombinant ASC (RecASC) activated NLRP3 and induced pro-inflammatory responses, exacerbating the outcome of ischemic stroke, in WT mice, but not in NLRP3 KOmice.