Data Management And Sharing System of China National Clinical Research Center for Neurological Diseases
Nitrated alpha-synuclein in minor salivary gland biopsies in Parkinson's disease
First author：Ma Ling-Yan,Gao Li-Yan
Corresponding author：Feng Tao
essential_information
- Person in charge：Ma Ling-Yan,Gao Li-Yan
- Data Sources： OTHERS
- Number of papers：OTHERS20190847
- Published time：2019-06-21
2019-06-21
Published articles
Journal：Neuroscience Letters
Publication type: Research Article
IF(IF): 3.046
PMID: 30946930 Link To Pubmed Full text Download
Background And Purpose
Method
Patients with PD and age-matched controls underwent minor salivary gland biopsy. Motor impairment was assessed by Hoehn-Yahr (H-Y) stage and Unified Parkinson's Disease Rating Scale (UPDRS) Part III in off-state. 11C-methyl-N-2b-carbomethoxy-3b-(4-fluorophenyl) tropane (11C-CFT) DAT-PET scan was performed in all subjects. Immunohistochemical staining for nitrated α-SYN was performed in the minor salivary gland tissues.
Conclusion
Our result suggests that nitrated α-SYN exists in the early stage and is probably a promising biomarker for PD. Minor salivary gland is an ideal site for α-SYN nitration detection. Despite of the small number of subjects, attention should be given to α-SYN nitration in PD and more investigations on nitrated α-SYN in different sites and large sample using should be explored in future.
Main results
The minor salivary gland tissues of 8 PD cases and 7 controls with early stage (H-Y stage 1-2) were detected. All PD patients showed asymmetrical and reduction of 11C-CFT uptake in the caudate, anterior and posterior putamen, while all control subjects showed normal DAT-PET scan. Positive nitrated α-SYN immunostaining was observed in all PD patients (8/8,100%) but not in control subjects (0/7). The results were consistent well with that of DAT-PET. These nitrated alpha-synuclein positive structures were mainly located in the periacinar stroma in PD patients.