Data Management And Sharing System of China National Clinical Research Center for Neurological Diseases
DNA Methylation Regulates Gene Expression in Intracranial Aneurysms
First author：Yu, Lanbing
Corresponding author：Zhao, Jizong
essential_information
- Person in charge：Yu, Lanbing
- Data Sources： OTHERS
- Number of papers：OTHERS20190557
- Published time：2017-09-01
2017-09-01
Published articles
Journal：World Neurosurg
Publication type: Original Article
IF(IF): 2.104
PMID: 28433851 Link To Pubmed Full text Download
Background And Purpose
Method
We compared 20 intracranial aneurysm tissue specimens with 20 matched specimens from the superficial temporal artery as controls. We identified the gene expression profiles in these samples using the GeneChip Human U133 Plus 2.0 array and evaluated DNA methylation modifications using the Infinium HumanMethylation450 BeadChip Kit.
Conclusion
In the development of intracranial aneurysms, DNA methylation plays an important role in the regulation of genetic expression involved in immune and inflammatory reactions, cell function, cell maintenance, and cell signal transduction.
Main results
A total of 11,022 differentially methylated sites between aneurysm tissues and matched control tissues were identified, and 2142 differentially expressed gene transcripts were detected based on the 2 gene expression profiles. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses and verification analysis showed that the MYH11, LIFR, and TLR4 genes were associated with the occurrence and development of intracranial aneurysms. These genes mainly encode cell adhesion molecules or are involved in the NF-κB, JAK-STAT, and ERK/JNK signaling pathways.