Data Management And Sharing System of China National Clinical Research Center for Neurological Diseases
Quantitative proteomics analysis of differentially expressed proteins in ruptured and unruptured cerebral aneurysms by iTRAQ
First author：Jiang Pengjun
Corresponding author：Wang Shuo
essential_information
- Person in charge：Jiang Pengjun
- Data Sources： OTHERS
- Number of papers：OTHERS20190660
- Published time：2018-06-30
2018-06-30
Published articles
Journal：J Proteomics
Publication type: Article
IF(IF): 4.044
PMID: 29729990 Link To Pubmed Full text Download
Background And Purpose
Method
This study was performed to investigate the differentially expressed proteins between ruptured and unruptured aneurysms using quantitative proteomics. The aneurysmal walls of six ruptured aneurysms and six unruptured aneurysms were collected during the surgical operation. The isobaric tags for relative and absolute quantification (iTRAQ) were used to identify the differentially expressed proteins and western blotting was performed to validate the expression of the proteins of interest. Bioinformatics analysis of the differentially expressed proteins was also performed using the KEGG database and GO database.
Conclusion
Through the iTRAQ method, we found that inflammatory responses and cell-matrix interactions may play a significant role in the rupture of cerebral aneurysms. These findings provide a basis for better understanding of pathophysiological mechanisms associated with aneurysm rupture.
Main results
Between ruptured and unruptured aneurysms, 169 proteins were found differently expressed, including 74 up-regulated proteins and 95 down-regulated proteins with a fold change ≥ 2 and p value ≤ .05. KEGG pathway analysis revealed that phagosome, focal adhesion and ECM-receptor interaction were the most common pathways involved in aneurysm rupture. In addition, the differential expressions of ITGB3, CRABP1 and S100A9 were validated by western blotting.