Data Management And Sharing System of China National Clinical Research Center for Neurological Diseases
In vitro characteristics of endothelial cells prepared from human cerebral arteriovenous malformation lesions using a novel method
First author：Hao Q., Q.
Corresponding author：Zhao Y. L., Y. L.
essential_information
- Person in charge：Hao Q., Q.
- Data Sources： OTHERS
- Number of papers：OTHERS20190687
- Published time：2017-10-28
2017-10-28
Published articles
Journal：Microvascular Research
Publication type: Article
IF(IF): 3.514
PMID: 29111303 Link To Pubmed Full text Download
Background And Purpose
Method
We developed a protocol for isolating and growing ECs from eight patients with cAVM. The tissues were microsurgically removed from cAVM lesion and were digested by 0.25% Trypsin-EDTA, and cultured in ECM medium. ECs were selected by FACS and confirmed their EC origin by immunocytochemistry of the basic expression patterns of CD31 and CD34. LDL-uptake and capillary tube formation were used to determine their functional features.
Conclusion
Using the protocol, isolated cAVM-ECs are stably established, and retain their endothelial phenotypes. These cAVM-ECs may provide a biological tool to examine molecular phenotypes and mechanisms responsible for human cAVM.
Main results
The isolated cAVM-ECs exhibited contact inhibition of growth and appearance of rounded cobblestone. cAVM-ECs were CD31- and CD34-positive. In functional assays, cAVM-ECs were able to uptake LDL and form capillary tubes. cAVM-ECs from younger patients proliferated faster than that from elders, and cAVM-ECs were less stable than normal artery ECs. In addition, cAVM-ECs appeared to more easily transform into mesenchymal cells than normal artery ECs.