国家神经系统疾病临床医学研究中心数据管理与共享系统
Data Management And Sharing System of China National Clinical Research Center for Neurological Diseases
Alpha-synuclein in erythrocyte membrane of patients with multiple system atrophy: A pilot study
通讯作者：Feng Tao
基本信息
- 负责人：Liu Genliang,Tian Chen
- 数据来源： OTHERS
- 论文编号：OTHERS20190796
- 发表时间：2019-03-01
2019-03-01
已发表文章
发表杂志：Parkinsonism & Related Disorders
发表类型: Research Article
影响因子(IF): 4.891
PMID: 30228038 Pubmed原文链接 全文下载
主要研究背景及目的
方法
The membrane and cytoplasm were extracted from erythrocytes in 77 patients with MSA and 133 healthy controls. Levels of total and oligomeric alpha-synuclein were detected using Electrochemiluminescence assays. The correlations between alpha-synuclein levels and clinical characteristics were explored in MSA group. The diagnostic value of erythrocyte alpha-synudein for MSA was determined by Receiver operator characteristic curve.
结论
Our pilot study suggests that the membrane fraction of alpha-synuclein levels in erythrocyte were elevated in patients with MSA, and these levels may be decreased with the development of disease.
主要结果
alpha-synuclein levels in the erythrocyte membrane were significantly elevated in MSA patients compared with the healthy controls (total alpha-synuclein, p = 0.003; oligomeric alpha-synuclein/total alpha-synuclein, p = 0.033; oligomeric alpha-synuclein/protein, p < 0.001). The combination of total and oligomeric alpha-synuclein levels in erythrocyte membrane could efficiently distinguish MSA from healthy controls (sensitivity of 79.2%; specificity of 69.2%; area under the curve: 0.771). In contrast, no significant difference was found in erythrocyte cytoplasm alpha-synuclein levels. In the subgroup of 48 patients with probable MSA, there was a weakly negative correlation between oligomeric alpha-synuclein/protein in erythrocyte membrane and disease duration (r = -0.336; p = 0.009).