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Alpha-synuclein in erythrocyte membrane of patients with multiple system atrophy: A pilot study

First author：Liu Genliang,Tian Chen

Corresponding author：Feng Tao

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essential_information

2019-03-01

Published articles

Journal：Parkinsonism & Related Disorders

Publication type: Research Article

IF(IF): 4.891

PMID: 30228038 Link To Pubmed Full text Download

Background And Purpose

Method

The membrane and cytoplasm were extracted from erythrocytes in 77 patients with MSA and 133 healthy controls. Levels of total and oligomeric alpha-synuclein were detected using Electrochemiluminescence assays. The correlations between alpha-synuclein levels and clinical characteristics were explored in MSA group. The diagnostic value of erythrocyte alpha-synudein for MSA was determined by Receiver operator characteristic curve.

Conclusion

Our pilot study suggests that the membrane fraction of alpha-synuclein levels in erythrocyte were elevated in patients with MSA, and these levels may be decreased with the development of disease.

Main results

alpha-synuclein levels in the erythrocyte membrane were significantly elevated in MSA patients compared with the healthy controls (total alpha-synuclein, p = 0.003; oligomeric alpha-synuclein/total alpha-synuclein, p = 0.033; oligomeric alpha-synuclein/protein, p < 0.001). The combination of total and oligomeric alpha-synuclein levels in erythrocyte membrane could efficiently distinguish MSA from healthy controls (sensitivity of 79.2%; specificity of 69.2%; area under the curve: 0.771). In contrast, no significant difference was found in erythrocyte cytoplasm alpha-synuclein levels. In the subgroup of 48 patients with probable MSA, there was a weakly negative correlation between oligomeric alpha-synuclein/protein in erythrocyte membrane and disease duration (r = -0.336; p = 0.009).