国家神经系统疾病临床医学研究中心数据管理与共享系统
Data Management And Sharing System of China National Clinical Research Center for Neurological Diseases
Overexpression of miR-224-3p alleviates apoptosis from cerebral ischemia reperfusion injury by targeting FIP200
第一作者：Deng Yiming
通讯作者：Gao Feng
基本信息
- 负责人：Deng Yiming
- 数据来源： OTHERS
- 论文编号：OTHERS20190816
- 发表时间：2019-05-27
2019-05-27
已发表文章
发表杂志：Journal of cellular biochemistry
发表类型: Research Article
影响因子(IF): 4.429
PMID: 31134677 Pubmed原文链接 全文下载
主要研究背景及目的
方法
Oxygen glucose deprivation model in N2a cells were used to perform the cerebral I/R injury in vitro. Trypan blue staining, reactive oxygen species (ROS) production, and caspase-3 were measured to evaluate the function of miR-224-3p.
结论
We evaluated the function and mechanism of miR-224-3p in ischemic brain injury. miR-224-3p may serve as a potential target for new therapeutic intervention.
主要结果
Overexpression of miR-224-3p alleviated the apoptosis induced by oxygen glucose deprivation (OGD) and cleaved caspase-3 was significantly reduced. We further provided the possible mechanism that miR-224-3p may protect cells from cerebral I/R injury by targeting FAK family-interacting protein (FIP200). Further rescue experiment proved that overexpression of FIP200 partially blocked the effect of miR-224-3p.