Data Management And Sharing System of China National Clinical Research Center for Neurological Diseases
Overexpression of miR-224-3p alleviates apoptosis from cerebral ischemia reperfusion injury by targeting FIP200
First author：Deng Yiming
Corresponding author：Gao Feng
essential_information
- Person in charge：Deng Yiming
- Data Sources： OTHERS
- Number of papers：OTHERS20190816
- Published time：2019-05-27
2019-05-27
Published articles
Journal：Journal of cellular biochemistry
Publication type: Research Article
IF(IF): 4.429
PMID: 31134677 Link To Pubmed Full text Download
Background And Purpose
Method
Oxygen glucose deprivation model in N2a cells were used to perform the cerebral I/R injury in vitro. Trypan blue staining, reactive oxygen species (ROS) production, and caspase-3 were measured to evaluate the function of miR-224-3p.
Conclusion
We evaluated the function and mechanism of miR-224-3p in ischemic brain injury. miR-224-3p may serve as a potential target for new therapeutic intervention.
Main results
Overexpression of miR-224-3p alleviated the apoptosis induced by oxygen glucose deprivation (OGD) and cleaved caspase-3 was significantly reduced. We further provided the possible mechanism that miR-224-3p may protect cells from cerebral I/R injury by targeting FAK family-interacting protein (FIP200). Further rescue experiment proved that overexpression of FIP200 partially blocked the effect of miR-224-3p.