国家神经系统疾病临床医学研究中心数据管理与共享系统
Data Management And Sharing System of China National Clinical Research Center for Neurological Diseases
基本信息
- 负责人：Cao Runjing,Li Shiping
- 数据来源： OTHERS
- 论文编号：OTHERS20190819
- 发表时间：2019-06-17
2019-06-17
已发表文章
发表杂志：Cell adhesion & migration
发表类型: Short Communication
影响因子(IF): 3.405
PMID: 31208274 Pubmed原文链接 全文下载
主要研究背景及目的
方法
We used a middle cerebral artery occlusion (MCAO) model of focal ischemia. We then applied lentivirus-packaged SIRT3 overexpression and knock down in microglial N9 cells to investigate the underlying mechanism driving microglial cell migration. More microglial cells appeared in the ischemic lesion side after MCAO.
结论
Our study for the first time shows that SIRT3 promotes microglia migration by upregulating CX3CR1.
主要结果
The levels of SIRT3 were increased in macrophages, the main source of microglia, after ischemia. CX3CR1 levels were increased with SIRT3 overexpression. SIRT3 promoted microglial N9 cells migration by upregulating CX3CR1 in both normal and glucose deprived culture media. These effects were G protein-dependent.