Data Management And Sharing System of China National Clinical Research Center for Neurological Diseases
Sirtuin 3 promotes microglia migration by upregulating CX3CR1
First author：Cao Runjing,Li Shiping
Corresponding author：Guo Li,Shi Jiong
essential_information
- Person in charge：Cao Runjing,Li Shiping
- Data Sources： OTHERS
- Number of papers：OTHERS20190819
- Published time：2019-06-17
2019-06-17
Published articles
Journal：Cell adhesion & migration
Publication type: Short Communication
IF(IF): 3.405
PMID: 31208274 Link To Pubmed Full text Download
Background And Purpose
Method
We used a middle cerebral artery occlusion (MCAO) model of focal ischemia. We then applied lentivirus-packaged SIRT3 overexpression and knock down in microglial N9 cells to investigate the underlying mechanism driving microglial cell migration. More microglial cells appeared in the ischemic lesion side after MCAO.
Conclusion
Our study for the first time shows that SIRT3 promotes microglia migration by upregulating CX3CR1.
Main results
The levels of SIRT3 were increased in macrophages, the main source of microglia, after ischemia. CX3CR1 levels were increased with SIRT3 overexpression. SIRT3 promoted microglial N9 cells migration by upregulating CX3CR1 in both normal and glucose deprived culture media. These effects were G protein-dependent.