国家神经系统疾病临床医学研究中心数据管理与共享系统
Data Management And Sharing System of China National Clinical Research Center for Neurological Diseases
alpha-Synuclein in salivary extracellular vesicles as a potential biomarker of Parkinson's disease
第一作者：Cao Zhentang
通讯作者：Feng Tao
基本信息
- 负责人：Cao Zhentang
- 数据来源： OTHERS
- 论文编号：OTHERS20190846
- 发表时间：2019-03-23
2019-03-23
已发表文章
主要研究背景及目的
方法
Saliva samples were obtained from 74 PD and 60 healthy controls (HCs). The EVs were extracted from saliva by XYCQ EV Enrichment KIT. Western blot and Nanosight 300 were used to validate the existence of exosomes in EVs and to analyze the size of salivary EVs. Salivary EVs alpha-syn levels, including total alpha-syn (alpha-syn(Total)), oligomeric alpha-syn (alpha-syn(Olig)) and phosphorylated-ser129 alpha-syn (alpha-syn(PS129)), were measured by Electrochemiluminescence (ECL) assays. Diagnostic value and clinical relevance of salivary EVs alpha-syn were assessed by Receiver Operator Characteristic (ROC) curve and Spearman correlation.
结论
Exosomes are present in PD saliva. The alpha-syn(Olig) and alpha-syn(Olig)/alpha-syn(Total) ratio in salivary EVs may serve as potential diagnostic biomarkers for PD.
主要结果
Alix and CD9 positive EVs, representing the presence of exosomes, was detected in PD salivary samples. Size of salivary EVs was about 30-400 nm. The levels of alpha-syn(Olig) and alpha-syn(Olig)/alpha-syn(Total) in the salivary EVs were higher in PD than in HCs (10.39 +/- 1.46 pg/ng vs.1.37 +/- 0.24 pg/ng, p < 0.001;1.70 +/- 0.52 pg/ng vs.0.67 +/- 0.26 pg/ng, p < 0.001). There was no significant difference in alpha-syn(Total), alpha-syn(PS129), alpha-syn(PS129)/alpha-syn(Total) ratio between PD and HCs (P = 0.723, 0.634, 0.734, respectively). alpha-syn(Olig), 2.05 pg/ng distinguished PD from HCs with sensitivity 92% and specificity 86%; alpha-syn(Olig)/alpha-syn(Total) 0.18 pg/ng differentiated PD from HCs with sensitivity 81% and specificity 71%. No significant correlation between salivary EVs alpha-syn(Olig), alpha-syn(Olig)/asyn(Total) and disease severity was found.