国家神经系统疾病临床医学研究中心数据管理与共享系统
Data Management And Sharing System of China National Clinical Research Center for Neurological Diseases
A comprehensive map and functional annotation of the normal human cerebrospinal fluid proteome.
第一作者：Yang Zhang, Zhengguang Guo
通讯作者：Wei Sun
基本信息
- 负责人：Yang Zhang, Zhengguang Guo
- 数据来源： OTHERS
- 论文编号：OTHERS20190310
- 发表时间：2015-02-07
2015-02-07
已发表文章
发表杂志：Journal of Proteomics
发表类型: Biochemical Research Methods
影响因子(IF): 4.044
PMID: 25661039 Pubmed原文链接 全文下载
主要研究背景及目的
方法
In this study, high-pH reverse-phase liquid chromatography (hp-RPLC) was first integrated with a TripleTOF 5600 mass spectrometer to comprehensively profile the normal CSF proteome. A total of 49,836 unique peptides and 3256 non-redundant proteins were identified. To obtain high-confidence results, 2513 proteins with at least 2 unique peptides were further selected as bona fide CSF proteins.
结论
Nearly 30% of the identified CSF proteins have not been previously reported in the normal CSF proteome. More than 25% of the CSF proteins were components of CNS cell microenvironments, and network analyses indicated their roles in the pathogenesis of neurological diseases. The top canonical pathway in which the CSF proteins participated was axon guidance signaling. More than one third of the CSF proteins (788 proteins) were related to neurological diseases, and these proteins constitute potential CSF biomarker candidates.
主要结果
Nearly 30% of the identified CSF proteins have not been previously reported in the normal CSF proteome. More than 25% of the CSF proteins were components of CNS cell microenvironments, and network analyses indicated their roles in the pathogenesis of neurological diseases. The top canonical pathway in which the CSF proteins participated was axon guidance signaling. More than one third of the CSF proteins (788 proteins) were related to neurological diseases, and these proteins constitute potential CSF biomarker candidates.