Data Management And Sharing System of China National Clinical Research Center for Neurological Diseases
Data for a comprehensive map and functional annotation of the human cerebrospinal fluid proteome
First author：Yang Zhang, Zhengguang Guo
Corresponding author：Yajie Wang, Wei Sun
essential_information
- Person in charge：Yang Zhang, Zhengguang Guo
- Data Sources： OTHERS
- Number of papers：OTHERS20190373
- Published time：2015-02-20
2015-02-20
Published articles
Journal：Data in Brief
Publication type: Original Article
IF(IF): 0
PMID: 26217727 Link To Pubmed Full text Download
Background And Purpose
Method
In this study, high-pH reverse-phase liquid chromatography (hp-RPLC) was first integrated with a TripleTOF 5600 mass spectrometer to comprehensively profile the normal CSF proteome.
Conclusion
The mapping results can be freely downloaded at http://122.70.220.102:8088/csf/, which can be used to navigate the CSF proteome. For more information about the data, please refer to the related original article [1], which has been recently accepted by Journal of Proteomics.
Main results
A total of 49,836 unique peptides and 3256 non-redundant proteins were identified. To obtain high-confidence results, 2513 proteins with at least 2 unique peptides were further selected as bona fide CSF proteins. Nearly 30% of the identified CSF proteins have not been previously reported in the normal CSF proteome. More than 25% of the CSF proteins were components of CNS cell microenvironments, and network analyses indicated their roles in the pathogenesis of neurological diseases. The top canonical pathway in which the CSF proteins participated was axon guidance signaling. More than one-third of the CSF proteins (788 proteins) were related to neurological diseases, and these proteins constitute potential CSF biomarker candidates.