Data Management And Sharing System of China National Clinical Research Center for Neurological Diseases
RNaseH2A is involved in human gliomagenesis through the regulation of cell proliferation and apoptosis.
First author：Bin Dai
Corresponding author：Liwei Zhang
essential_information
2016-07-01
Published articles
Journal：Oncology Reports
Publication type: Research Article
IF(IF): 3.906
PMID: 27176716 Link To Pubmed Full text Download
Background And Purpose
Method
RNaseH2A was silenced in glioblastoma cell lines U87 and U251. Gene expression was assessed in the cells transfected with RNaseH2A shRNA or scramble shRNA by microarrays, validated by quantitative real time PCR. Protein expression was evaluated by western blot analysis. Cell proliferation was assessed by the MTT assay; cell cycle distribution and apoptosis were analyzed by flow cytometry. Finally, the effects of RNaseH2A on colony formation and tumorigenicity were assessed in vitro and in a mouse xenograft model, respectively.
Conclusion
RNaseH2A may be involved in human gliomagenesis, likely by regulating signaling pathways responsible for cell proliferation and apoptosis.