Data Management And Sharing System of China National Clinical Research Center for Neurological Diseases
The Landscape of Viral Expression Reveals Clinically Relevant Viruses with Potential Capability of Promoting Malignancy in Lower-Grade Glioma
First author：Wang, Zheng Hao, Yajing Zhang, Chuanbao
Corresponding author：Zhou, Dabiao Chen, Runsheng Jiang, Tao
essential_information
- Person in charge：Wang, Zheng Hao, Yajing Zhang, Chuanbao
- Data Sources： OTHERS
- Number of papers：OTHERS20190471
- Published time：2017-05-01
2017-05-01
Published articles
Journal：Clin Cancer Res
Publication type: Research Article
IF(IF): 12.531
PMID: 27864420 Link To Pubmed Full text Download
Background And Purpose
Method
In total, 325 samples were enrolled into this study. Reads that failed to map to the human genome were aligned to viral genomes and screened for potential virus-derived transcripts. For quantification, VPKM was calculated according to mapped reads weighted by genome sizes and sequencing depth.
Conclusion
RNA-seq was sufficient to identify virus infection in glioma samples. SV40 and WMSV were identified to be prognostic markers for patients with lower-grade gliomas and showed potential values for targeting therapy. Clin Cancer Res; 23(9); 2177-85. ©2016 AACR.
Main results
We observed that viruses tended to concertedly express in a certain subgroup of patients. Survival analysis revealed that individuals who were infected with Simian virus 40 (SV40) or woolly monkey sarcoma virus (WMSV) had a significantly shorter overall survival than those uninfected. A multivariate Cox proportional hazards model, taking clinical and molecular factors into account, was applied to assess the prognostic value of SV40 and WMSV. Both SV40 and WMSV were independent prognostic factors for predicting patient's survival in lower-grade gliomas. Subsequent gene analysis demonstrated that SV40 was correlated with regulation of transcription, whereas WMSV was correlated with cell-cycle phase, which indicated frequent proliferation of tumor cells.