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The interaction between calcineurin and alpha-synuclein is regulated by calcium and calmodulin
First author：Shi Xiaoyu,Sun Yue, Yue
Corresponding author：Li Zhimei,Luo Jing, Jing
essential_information
- Person in charge：Shi Xiaoyu,Sun Yue, Yue
- Data Sources： OTHERS
- Number of papers：OTHERS20190685
- Published time：2018-02-19
2018-02-19
Published articles
Journal：Biochem Biophys Res Commun
Publication type: Research Article
IF(IF): 2.705
PMID: 29409956 Link To Pubmed Full text Download
Background And Purpose
Method
Based on the data from microscale thermophoresis, GST pull-down assays, and co-immunoprecipitation, we found that CN binds α-syn. Furthermore, this interaction is mediated by calcium/calmodulin (Ca2+/CaM) signaling. Additionally, thapsigargin (TG) triggered an increase in CN activity and α-syn aggregation in HEK293 cells stably transfected with α-syn.
Conclusion
hese in vivo and in vitro studies have been complementary with each other, representing the changes in the CN-dependent pathway affected by overexpression of α-syn.
Main results
Our previous study in vivo suggest that overexpression of α-syn in transgenic mice significantly promoted CN activity and subsequent nuclear translocation of nuclear factor of activated T-cells (NFAT) in the midbrain dopaminergic (mDA) neurons.