国家神经系统疾病临床医学研究中心数据管理与共享系统
Data Management And Sharing System of China National Clinical Research Center for Neurological Diseases
The interaction between calcineurin and alpha-synuclein is regulated by calcium and calmodulin
基本信息
- 负责人：Shi Xiaoyu,Sun Yue, Yue
- 数据来源： OTHERS
- 论文编号：OTHERS20190685
- 发表时间：2018-02-19
2018-02-19
已发表文章
发表杂志：Biochem Biophys Res Commun
发表类型: Research Article
影响因子(IF): 2.705
PMID: 29409956 Pubmed原文链接 全文下载
主要研究背景及目的
方法
Based on the data from microscale thermophoresis, GST pull-down assays, and co-immunoprecipitation, we found that CN binds α-syn. Furthermore, this interaction is mediated by calcium/calmodulin (Ca2+/CaM) signaling. Additionally, thapsigargin (TG) triggered an increase in CN activity and α-syn aggregation in HEK293 cells stably transfected with α-syn.
结论
hese in vivo and in vitro studies have been complementary with each other, representing the changes in the CN-dependent pathway affected by overexpression of α-syn.
主要结果
Our previous study in vivo suggest that overexpression of α-syn in transgenic mice significantly promoted CN activity and subsequent nuclear translocation of nuclear factor of activated T-cells (NFAT) in the midbrain dopaminergic (mDA) neurons.