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Auricular vagus nerve stimulation promotes functional recovery and enhances the post-ischemic angiogenic response in an ischemia/reperfusion rat model.
First author：Ying Jiang
Corresponding author：Changqing Li, Tao Feng
essential_information
- Person in charge：Ying Jiang
- Data Sources： OTHERS
- Number of papers：OTHERS20190388
- Published time：2016-07-01
2016-07-01
Published articles
Journal：Neurochemistry International
Publication type: Research Article
IF(IF): 3.921
PMID: 26964767 Link To Pubmed Full text Download
Background And Purpose
Method
Adult male Sprague-Dawley (SD) rats underwent transient middle cerebral artery occlusion (tMCAO) surgery and received repeated electrical stimulation of the left cavum concha starting 30 minutes after ischemia. For the following 21 days, we evaluated functional recovery at different time points using neurological deficit scores, the beam-walking test and the staircase test. The infarct volume was measured using TTC staining at 24 h post reperfusion, neuronal survival in the ischemic penumbra was assessed using hematoxylin and eosin (HE) staining. Microvessel density and endothelial cell proliferation in the ischemic boundary were assessed using immunofluorescence. The expression levels of brain-derived neurotrophic factor (BDNF), endothelial nitric oxide synthase (eNOS) and vascular endothelial growth factor (VEGF) in the ischemic penumbra were also evaluated.
Conclusion
Our findings suggest that repeated aVNS promoted post-ischemic functional recovery and angiogenesis, possibly in conjunction with the up-regulated expression of BDNF, eNOS and VEGF in the rat brain.