国家神经系统疾病临床医学研究中心数据管理与共享系统

Data Management And Sharing System of China National Clinical Research Center for Neurological Diseases

《国家中心数据管理与共享系统操作说明书 —— 研究者部分》

Auricular vagus nerve stimulation promotes functional recovery and enhances the post-ischemic angiogenic response in an ischemia/reperfusion rat model.

第一作者：Ying Jiang

通讯作者：Changqing Li, Tao Feng

auricular vagus nerve stimulation; BDNF; eNOS; VEG

基本信息

  • 负责人：Ying Jiang
  • 数据来源： OTHERS
  • 论文编号：OTHERS20190388
  • 发表时间：2016-07-01

2016-07-01

已发表文章

发表杂志：Neurochemistry International

发表类型: Research Article

影响因子(IF): 3.921

PMID: 26964767 Pubmed原文链接 全文下载

主要研究背景及目的

方法

Adult male Sprague-Dawley (SD) rats underwent transient middle cerebral artery occlusion (tMCAO) surgery and received repeated electrical stimulation of the left cavum concha starting 30 minutes after ischemia. For the following 21 days, we evaluated functional recovery at different time points using neurological deficit scores, the beam-walking test and the staircase test. The infarct volume was measured using TTC staining at 24 h post reperfusion, neuronal survival in the ischemic penumbra was assessed using hematoxylin and eosin (HE) staining. Microvessel density and endothelial cell proliferation in the ischemic boundary were assessed using immunofluorescence. The expression levels of brain-derived neurotrophic factor (BDNF), endothelial nitric oxide synthase (eNOS) and vascular endothelial growth factor (VEGF) in the ischemic penumbra were also evaluated.

结论

Our findings suggest that repeated aVNS promoted post-ischemic functional recovery and angiogenesis, possibly in conjunction with the up-regulated expression of BDNF, eNOS and VEGF in the rat brain.

主要结果